Journal: Pharmaceutics

Article Title: PLGA Microparticles as a Stable and Biocompatible Carrier for Adiponectin Delivery to Enhance Bone Regeneration

doi: 10.3390/pharmaceutics18050546

Figure Lengend Snippet: Evaluate the biocompatibility of ADPN-MPs with bone cells. ( A ) The CCK-8 method to evaluate the biocompatibility of ADPN-MPs with MC3T3-E1 cells; ( B ) The MTT method to evaluate the biocompatibility of ADPN-MPs with BMSCs. (Comparison among the three groups, * p < 0.05, **** p < 0.0001, ns means not significant).

Article Snippet: Monolayer adherent Mouse Calvaria Osteoblast Precursor Cells (MC3T3-E1) (ATCC ® CRL-2593TM, passage 5) in the logarithmic growth phase were seeded into a 96-well plate at a density of 5 × 10 3 cells per well and incubated for 1, 4, and 7 days.

Techniques: CCK-8 Assay, Comparison

Journal: Gels

Article Title: Whey Protein Isolate Hydrogels Containing Cannabidiol Support the Proliferation of Pre-Osteoblasts

doi: 10.3390/gels11060418

Figure Lengend Snippet: The results of % cell viability and proliferation of MC3T3-E1 pre-osteoblasts seeded on WPI/CBD hydrogels relative to WPI0 (set as 100%). TCPS is tissue-culture-treated polystyrene and serves as control. Absorbance readings at 570/600 nm were taken at days 3 and 7. Each bar represents the mean ± SD of n = 6 (** p < 0.01, **** p < 0.0001; compared to the WPI control) ( ## p < 0.01, #### p < 0.0001; compared to the TCPS control).

Article Snippet: Cellular viability and behaviour assays were conducted utilising the mouse calvaria osteoblast precursor cell line (MC3T3-E1, DSMZ Braunschweig, Germany, ACC-210).

Techniques: Control

Journal: Gels

Article Title: Whey Protein Isolate Hydrogels Containing Cannabidiol Support the Proliferation of Pre-Osteoblasts

doi: 10.3390/gels11060418

Figure Lengend Snippet: SEM images demonstrating the cellular morphology of MC3T3-E1 pre-osteoblasts on WPI/CBD hydrogels. Images were taken at 7 and 10 days of culture ( middle and lower panels ). The upper panel shows unseeded hydrogels (without cells) to facilitate comparison. Scale bars represent 10 μm.

Article Snippet: Cellular viability and behaviour assays were conducted utilising the mouse calvaria osteoblast precursor cell line (MC3T3-E1, DSMZ Braunschweig, Germany, ACC-210).

Techniques: Comparison

Journal: Gels

Article Title: Whey Protein Isolate Hydrogels Containing Cannabidiol Support the Proliferation of Pre-Osteoblasts

doi: 10.3390/gels11060418

Figure Lengend Snippet: Assessment of the osteogenic potential of MC3T3-E1 pre-osteoblastic cells cultured on WPI/CBD hydrogels; ( a ) the expression of normalised ALP-specific activity on days 3, 7, and 14, ( b ) collagen production, and ( c ) calcium production by pre-osteoblasts on days 7, 14, and 21. Each bar represents the mean ± SD of n = 6 (* p < 0.05, ** p < 0.01, **** p < 0.0001; compared to the WPI0 control) ( # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001; compared to the TCPS control), while the absence of asterisks in the graph of calcium production ( c ) signifies statistically non-significant differences when compared to the WPI0 control.

Article Snippet: Cellular viability and behaviour assays were conducted utilising the mouse calvaria osteoblast precursor cell line (MC3T3-E1, DSMZ Braunschweig, Germany, ACC-210).

Techniques: Cell Culture, Expressing, Activity Assay, Control